Protocols
Rag2 genotyping PCR protocol (link)
Commassie Staining Protocol (January 2008)
Retroviral Transduction of B cells (January 2008)
Western Blot (link)
Purification of antibodies (January 2008)
Elispot
Immunization
Immunostaining
Recovery of plasmids from yeast
Sorting of single cells
Sorting of single cells
Preparation of competent cells using CaCl2 (link)
CaCl2 Competent cell stock protocol
Transform plasmid to competent cells
Transformation of bacteria (link)
Tissue Culture Methods (link)
Centrifuge RCF/RPM calculator (link1)
Centrifuge RCF/RPM calculator (link2)
10X PBS solution preparation (link)
RBC lysis Buffer preparation (link)
Stock Solutions
Primer Design (link1)
Primer Design (link2)
Sequence Extractor (link)
The Sequence Manipulation Suite (link)
DNA extraction Protocol (link)
Oligos to order
PHA Clones DB
SNP recommended alphabetical symbolism
addgene (link)
Heat Inactivation of Serum
Glycerol Bacterial Stocks
Infection of in vitro stimulated mouse B cells
DNA extraction from blood cells
Isolating DNA from Mouse Tail Clips + Lysis Buffer
TAE Buffer 50 X
Analysis of Real-time Q PCR data by the 2^-DDct method
AID cytidine deaminse activity assay
WHOLE BLOOD FACS STAINING FROM TAIL BLOOD (link)
